Abstract:
【Objective】 To analyze whether some transcription factors in
Arabidopsis thaliana, known for the secondary cell wall thicken, could regulate the expression of the key genes of xylosyltransferase, such as
FRA8,
IRX9,
IRX10,
IRX14,
F8H,
IRX9-L,
IRX10-L and
IRX14-L, and observe the phenotype of
KNAT7 dominant repression plant.【Method】 Effectors and reporters were constructed by Gateway Technology and the transient transcriptional activation assay was conducted. Construct pCAMBIA1304-p35S∷
KNAT7-SRDX recombinant plasmid by Gateway Technology and transform this plasmid into wild
A. thaliana via
Agrobacterium tumefaciens-floral dip method.【Result and conclusion】 The transient transcriptional activation assay revealed that transcription factors KNAT7, MYB46, ERF72, SND1, NST2 could activate the expression of a number of the key genes of xylosyltransferase. KNAT7 could activate the expression of
FRA8,
IRX9 and
IRX14-L. Furthermore, dominant repression of
KNAT7 significantly affected the growth of
Arabidopsis thaliana. These results indicate that
KNAT7 probably plays an important role in the regulation of xylan biosynthesis.