鬼臼毒素及4′- 去甲基鬼臼毒素对黄曲条跳甲的拒食活性研究
Antifeedant activities of podophyllotoxin and 4′-demethylpodophyotoxin against the adult of striped flea beetles,Phyllotreta striolata
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摘要: 【目的】研究鬼臼毒素及其类似物对黄曲条跳甲 Phyllotreta striolata 的拒食活性.【方法】从窝儿七 Rhizoma et Radix Diphylleiae Diphylleia sinensis Li提取分离得到鬼臼毒素和4′-去甲基鬼臼毒素，并分别配制成500、1 000、2 000、3 000 和 4 000 μg·mL-1 5种质量浓度，喂食黄曲条跳甲，比较不同浓度化合物在不同处理时间下对黄曲条跳甲的拒食率.【结果和结论】鬼臼毒素对黄曲条跳甲有显著的选择性拒食作用，且有一定持效性；4′-去甲基鬼臼毒素只在高质量浓度(4 000 μg·mL-1)下表现出选择性拒食活性，其他质量浓度无明显拒食作用.从两者分子结构推测鬼臼毒素母核上的甲氧基是拒食活性的决定基团.
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关键词:
- 窝儿七 /
- 鬼臼毒素 /
- 4′-去甲基鬼臼毒素 /
- 黄曲条跳甲 /
- 拒食活性
Abstract: 【Objective】Antifeedant activities of podophyllotoxin and 4′-demethylpodophyllotoxin against the adult of stried leaf beetles Phyllotreta striolata were studied. 【Method】Podophyllotoxin and 4′-demethylpodophyllotoxin were extracted and separated from Chinese umbrellaleaf rhizome. The antifeedant activities of podophyllotoxin and 4′-demethylpodophyllotoxin against P. striolata were made at 500, 1 000, 2 000, 3 000 and 4 000 μg·mL-1 concentrations for 24 h and 48 h respectively.【Result and conclusion】Podophyllotoxin showed a selective antifeedant effect, and the activities remained unchanged as time extended. By contrast, 4′-demethylpodophyllotoxin had no obvious influence on antifeedant activities except under high concentration (4 000 μg·mL-1). The indexes of 4′-methyl were speculated to be the key functional group to affect the antifeedant activities according to the molecular structures of podophyllotoxin and 4′-hydroxypodophyllotoxin. -
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