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    彭练慈, 殷中琼, 贾仁勇, 李莉, 代如意, 曲径, 刘明辉, 陈萍. 20 味中药提取物对无乳链球菌的体外抑菌活性研究[J]. 华南农业大学学报, 2014, 35(4): 22-25. DOI: 10.7671/j.issn.1001-411X.2014.04.005
    引用本文: 彭练慈, 殷中琼, 贾仁勇, 李莉, 代如意, 曲径, 刘明辉, 陈萍. 20 味中药提取物对无乳链球菌的体外抑菌活性研究[J]. 华南农业大学学报, 2014, 35(4): 22-25. DOI: 10.7671/j.issn.1001-411X.2014.04.005
    shu
    PENG Lianci, YIN Zhongqiong, JIA Renyong, LI li, DAI Ruyi, QU Jing, LIU Minghui, CHEN Ping. Effects of twenty traditional Chinese medicine extracts against Streptococcus agalactiae in vitro[J]. Journal of South China Agricultural University, 2014, 35(4): 22-25. DOI: 10.7671/j.issn.1001-411X.2014.04.005
    Citation: PENG Lianci, YIN Zhongqiong, JIA Renyong, LI li, DAI Ruyi, QU Jing, LIU Minghui, CHEN Ping. Effects of twenty traditional Chinese medicine extracts against Streptococcus agalactiae in vitro[J]. Journal of South China Agricultural University, 2014, 35(4): 22-25. DOI: 10.7671/j.issn.1001-411X.2014.04.005
    shu

    20 味中药提取物对无乳链球菌的体外抑菌活性研究

    Effects of twenty traditional Chinese medicine extracts against Streptococcus agalactiae in vitro

      摘要
    • 摘要: 【目的】 筛选对无乳链球菌 Streptococcus agalactiae 具有良好体外抑菌效果的中药.【方法】 通过乙醇回流、超声波方法提取20味中药的抗菌活性部位，采用试管二倍稀释法测定黄连 Coptis chinensis 等20味中药提取物对无乳链球菌的最低抑菌浓度（Minimal inhibitory concentration，MIC）和最低杀菌浓度（Minimal bactericidal concentration，MBC），并评价抗菌活性较强的几味中药的体外联合抑菌效果.【结果和结论】 乌梅Prunus mume、黄连、黄芩Scutellaria baicalensis、虎杖Polygonum cuspidatum和地榆Sanguisorba officinalis 5味中药提取物对无乳链球菌的MIC范围为7.80～31.25 mg/mL.蒲公英Taraxacum mongolicum、茵陈Artemisia scoparia、秦皮Fraxinus rhynchophylla、艾叶Artemisia argyi、黄柏Phellodendron chinense、鱼腥草Houttuynia cordata、紫花地丁Violae yedoensis和白头翁Pulsatilla chinensis 8味中药提取物的MIC范围为62.50～125.00 mg/mL.何首乌Po. multiflorum、苦参Sophora flavescens、穿心莲Andrographis paniculata、杜仲Eucommia ulmoides、连翘Forsythia suspensa、金银花Lonicera japonica和夏枯草Prunella vulgaris 7味中药提取物的MIC范围为250.00～500.00 mg/mL.联合抑菌试验结果表明，黄芩、虎杖、地榆和黄连的联合抑菌指数（FICI）≤1，虎杖、黄芩和地榆的FICI＞2.黄连、黄芩、虎杖和地榆对无乳链球菌均具有很强的体外抗菌活性，地榆、黄连为相加作用，黄连、黄芩和虎杖为协同作用，地榆与黄芩、虎杖为拮抗作用.

       

      Abstract: 【Objective】 Screening for the traditional Chinese medicines which have good antibacterial effects on Streptococcus agalactiae in vitro.【Method】 Using ethanol reflux and ultrasonic method, extracts of 20 different traditional Chinese medicines were obtained. Minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of Coptis chinensis and other 19 extracts of traditional Chinese medicines on St. agalactiae were detected by macrodilution broth method, and combined antibacterial effects in vitro of them were also observed. 【Result and conclusion】 The MICs of the extracts obtained from Prunus mume, Coptis chinensis, Scutellaria baicalensis, Polygonum cuspidatum and Sanguisorba officinalis against St. agalactiae ranged from 7.80 to 31.25 mg/mL. The MICs of the extracts obtained from Taraxacum mongolicum, Artemisia scoparia, Fraxinus rhynchophylla, Artemisia argyi, Phellodendron chinense, Houttuynia cordata, Violae yedoensis and Pulsatilla chinensis ranged from 62.50 to 125.00 mg/mL. The MICs of the extracts obtained from Po. multiflorum, Sophora flavescens, Andrographis paniculata, Eucommia ulmoides, Forsythia suspensa, Lonicera japonica and Prunella vulgaris ranged from 250.00 to 500.00 mg/mL. The results of joint bacteriostatic test showed that the fractional inhibitory concentration index (FICI) of the extracts obtained from Sc. baicalensis, Po. cuspidatum, Sa. officinalisand C. chinensis was less than or equal to 1. The FICI of Po. cuspidatum, Sc. baicalensis and Sa. officinalis was more than 2. C. chinensis, Sc. baicalensis, Po. cuspidatum and Sa. officinalis showed a good antibacterial effect against St. agalactiae in vitro. The combination of Sa. officinalis and C. chinensis was characterized by a additive effect, whereas the combination of C. chinensis, Sc. baicalensis and Po. cuspidatum was characterized by a synergism effect. The combination of Sa. officinalis and Sc. baicalensis, Po. cuspidatum showed antagonism effect.

       

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