Abstract: A microwave digestion, hydride generation-atomic fluorescence spectrometer method for determining the total mercury residues of mercurous chloride, mercurous nitrate and mercuric acetate in animal derived food was established. The samples were pretreated with microwave digestion and determination by hydride generation-atomic fluorescence spectrometer, with the potassium borohydride as the reductant, and the hydrochloric acid as current-carrying. The density of mercury and its response value was in good linearity over the range of 0.05-2.00 μg/L(r>0.9990). As for mercurous chloride, mercurous nitrate and mercuric acetate, the limit of detection was 0.004, 0.001 and 0.006 μg/L respectively, the limit of quantification was 0.010,0.003 and 0.020 μg/L respectively, the minimum detectable concentration was 1.00, 0.25 and 1.50 μg/kg respectively, the relative standard deviation (RSD) was 3.82%, 3.27% and 4.11% respectively. The average recovery was 89.07%-110.08%, 80.17%-113.10% and 89.83%-112.77% respectively.