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    丁唯嘉, 陈洁怡, 王锦华, 李春远, 林永成, 周世宁. 2株红树内生真菌共培养产生的甾醇类化合物的分离鉴定[J]. 华南农业大学学报, 2013, 34(2): 267-271. DOI: 10.7671/j.issn.1001-411X.2013.02.027
    引用本文: 丁唯嘉, 陈洁怡, 王锦华, 李春远, 林永成, 周世宁. 2株红树内生真菌共培养产生的甾醇类化合物的分离鉴定[J]. 华南农业大学学报, 2013, 34(2): 267-271. DOI: 10.7671/j.issn.1001-411X.2013.02.027
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    DING Weijia, CHEN Jieyi, WANG Jinhua, LI Chunyuan, LIN Yongcheng, ZHOU Shining. Isolation and Identification of Sterols Produced by Two Mangrove Endophytic Fungi in Competing Co-culture[J]. Journal of South China Agricultural University, 2013, 34(2): 267-271. DOI: 10.7671/j.issn.1001-411X.2013.02.027
    Citation: DING Weijia, CHEN Jieyi, WANG Jinhua, LI Chunyuan, LIN Yongcheng, ZHOU Shining. Isolation and Identification of Sterols Produced by Two Mangrove Endophytic Fungi in Competing Co-culture[J]. Journal of South China Agricultural University, 2013, 34(2): 267-271. DOI: 10.7671/j.issn.1001-411X.2013.02.027
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    2株红树内生真菌共培养产生的甾醇类化合物的分离鉴定

    Isolation and Identification of Sterols Produced by Two Mangrove Endophytic Fungi in Competing Co-culture

      摘要
    • 摘要: 将来自南海红树林的内生真菌E33和K38混合发酵共培养,从菌丝体中分离到9种代谢产物，利用波谱技术确定它们为：24-甲基胆甾-5,24(28)-二烯-3β,15β, 19-三醇(化合物1)、24-甲基胆甾-5,24(28)-二烯-3β,19-二醇(化合物2)、3β,11α-二羟基麦角甾-8,24(28)-二烯-7-酮 (化合物3)、(22E, 24R)-麦角甾-5, 22-二烯-3β-羟基-7-酮(化合物4)、(22E, 24R)-6β-甲氧基麦角甾-7, 22-二烯-3β, 5αα, 6α-环氧麦角甾-8 (9) 22-二烯-3β, 7α-二醇(化合物6)及麦角甾醇、过氧化麦角甾醇和(22E, 24R)-麦角甾-7, 22-二烯-3β,5α,6β-三醇,其中化合物1、2为首次从海洋红树林真菌里分离得到.化合物1~6以往未从单独发酵的两菌中分离得到.

       

      Abstract: Two mangrove endophytic fungi K38 and E33 from the South China Sea Coast were chosen to be co-clutured. Nine sterols, 24-methylcholesta-5,24(28)-diene-3β,15β,19-triol（compound 1）,24-methylcholesta-5, 24(28)-diene-3β,19-diol (compound 2), 3β,11α-dihydroxyergosta-8, 24(28)-diene-7-one(compound 3),(22E, 24R)-ergosta-5, 22-diene-3β-hydroxyl-7-one (compound 4), (22E, 24R)-6β-methoxyergosta-5, 22-diene-3β, 5α-diol（compound 5）, (22E, 24R)-5α, 6α-epoxyergosta-8, 22-diene-3β,7α-diol(compound 6), ergosterol, ergosterol peroxide and (22E, 24R)-ergosta-7, 22-diene-3β, 5α, 6β-triol were isolated from the crude extracts of mycelia. Among them, compound 1 and compound 2 were found in marine mangrove fungi for the first time, and compounds 1~6 were not obtained from these two strains by previous pure fermentation.

       

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