摘要:
在模拟养殖条件下,对杂交鳢（斑鳢 Channa maculata♀×乌鳢 Channa argus♂）体内呋喃西林代谢物氨基脲（Semicarbazide,SEM）的残留及消除规律进行了研究.在（30.5±0.5）℃水温条件下,用质量浓度为2 mg/L呋喃西林药浴杂交鳢苗种2 d.药浴期间于0.5、1、2、4、8、12、24、48 h取样,停止药浴后于0.5、1、2、4、8、12、24、48、96、192、288、384、480、720、960、1 200、1 440、1 920、2 400、2 880 h取样,采用液-质联用（HPLC-MS）方法测定杂交鳢肌肉中SEM的含量.结果显示,药浴期间 0.5～48 h,肌肉中SEM质量分数从（32.58±2.82）μg/kg逐渐升高至（175.92±18.49）μg/kg；药浴结束后的SEM质量分数经测定于1 h达到最高,为（108.00±5.86）μg/kg；药浴结束后2～48 h,苗种体内SEM质量分数维持在（53.60±4.90）～（69.21±5.71）μg/kg,48 h后显著下降,至60 d后已低于检测限0.25 μg/kg.鱼体肌肉中SEM的平均消除速率为0.089 μg/（kg·h）.结果显示,在该试验条件下,鱼体内SEM可在60 d后代谢消除.
关键词:
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呋喃西林
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代谢物
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杂交鳢
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残留
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消除
Abstract:
In this paper, the residues and depuration of semicarbazide(SEM) in hybrid snakeheads（Channa maculata(♀)×Channa argus(♂)）were tested. Hybrid snakeheads were drug-bathed 2 days with 2 mg/L nitrofurazone at temperatures of (30.5±0.5) ℃.During drug-bath, the samples were collected at 0.5,1,2,4,8,12,24,48 h,and at 0.5,1,2,4,8,12,24,48,96,192,288,384,480,720,960,1 200,1 440,1 920,2 400,2 880 h after drug-bath, and the SEM residues in muscle were measured by high performance liquid chromatography tandem mass spectrometry(HPLC-MS).The results showed that the SEM residue from 0.5 to 48 h in hybrid snakehead muscle increased gradually from （32.58±2.82） to（175.92±18.49）μg/kg during drug-bath. SEM residue was found to be the highest,（108.00 ±5.86）μg/kg at 1 h after drug-bath. The residues ranged between（53.60±4.90）and（69.21±5.71）μg/kg from 2 to 48 h after drug-bath but significantly decreased after 48 h, which was below the detection limit of 0.25 μg/kg at 1 440 h. The average degradation of SEM in muscle was 0.089 μg/（kg·h）. The results showed that the nitrofurazone of muscle in this experimental condition was degraded after 60 days.
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