摘要:
以河西绒山羊、陇东绒山羊、辽宁绒山羊和内蒙古绒山羊为研究对象,采用PCR-SSCP和测序技术对Pit-1 基因第6外显子进行多态性研究,并分析该基因多态性与山羊体质量和体尺性状的关系.结果表明：Pit-1基因第6外显子存在2个SNPs位点并形成AA、AB、BB、AC和BC 5种基因型,BB为优势基因型.除内蒙古绒山羊外,其他3个山羊群体的基因型分布处于Hardy-Weinberg平衡状态.最小二乘法分析表明,AB基因型个体的体质量、胸围显著高于BC基因型（P<0.05）,其他基因型间差异不显著（P>0.05）. Pit-1基因是山羊生产育种应用中的一个有效遗传标记.
Abstract:
Genetic polymorphisms of Pit-1 gene was analyzed by sequencing and PCR-SSCP in Hexi cashmere goat, Longdong cashmere goat, Liaoning cashmere goat and Inner Mongolia cashmere goat, and the effects of genotype on body mass and body measurement traits of goat were also estimated in this study. The results showed that two SNPs（G/T, T/C）, and five genotypes（AA, AB, BB, AC and BC）were detected in exon 6, and the BB was the predominant genotype. The genotype distributions of four populations were all in the Hardy-Weinberg equilibrium except for Inner Mongolia cashmere goat. The least square analysis indicated that body mass and heart girth of individuals with genotype AB were significantly higher than that of individuals with genotype BC（P<0.05）, and the other genotypes were no significant difference（P>0.05）. Therefore, it can be concluded that genotypes of Pit-1 gene is an effective genetic marker in goat breeding.
下载:
