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    冯宏, 李永涛, 张干, 罗春玲. 强抗镉真菌的分离鉴定及溶磷能力研究[J]. 华南农业大学学报, 2013, 34(2): 177-181. DOI: 10.7671/j.issn.1001-411X.2013.02.010
    引用本文: 冯宏, 李永涛, 张干, 罗春玲. 强抗镉真菌的分离鉴定及溶磷能力研究[J]. 华南农业大学学报, 2013, 34(2): 177-181. DOI: 10.7671/j.issn.1001-411X.2013.02.010
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    FENG Hong, LI Yongtao, ZHANG Gan, LUO Chunling. Screening, Identification of Fungi with High Cadmium-Resistance and its Solubilization Capacity of Insoluble Phosphates[J]. Journal of South China Agricultural University, 2013, 34(2): 177-181. DOI: 10.7671/j.issn.1001-411X.2013.02.010
    Citation: FENG Hong, LI Yongtao, ZHANG Gan, LUO Chunling. Screening, Identification of Fungi with High Cadmium-Resistance and its Solubilization Capacity of Insoluble Phosphates[J]. Journal of South China Agricultural University, 2013, 34(2): 177-181. DOI: 10.7671/j.issn.1001-411X.2013.02.010
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    强抗镉真菌的分离鉴定及溶磷能力研究

    Screening, Identification of Fungi with High Cadmium-Resistance and its Solubilization Capacity of Insoluble Phosphates

      摘要
    • 摘要: 通过在培养基中加入200 mg/L镉（3CdSO4·8H2O）,从电子垃圾污染区土壤中筛选到1株强抗镉真菌,经ITS序列鉴定为月状旋孢腔菌Cochliobolus lunatus.该菌株可在镉质量浓度为2 000 mg/L的PDA平板上生长良好,抗性试验结果表明,菌株对镉的抗性可能与该菌在生长过程中产生碱性物质有关；菌株对镉、铅、锌和铜的最小抑菌浓度(MIC)均超过500 mg/L,菌体对9种重金属离子抗性的强弱顺序大致为：Zn2+、Cd2+>Pb2+>Cu2+>Co2+>Ni2+、Cr2+>Hg2+、Ag+.该菌株对3种难溶性的磷酸盐具有溶磷作用,溶磷能力强弱顺序为：Ca3(PO4)2(104 mg/L)>AlPO4 (86 mg/L)>FePO4·4 H2O (17 mg/L).

       

      Abstract: A fungus with high cadmium resistance was isolated from the soil polluted by e-waste primitive recycling activity by adding definite concentration of Cd (200 mg/L) as 3CdSO4·8H2O in the medium. The fungus belonged to Cochliobolus lunatus according to ITS rDNA sequences. This strain was able to grow well on PDA plate containing 2 000 mg/L Cd2+, and its cadmium resistance might be ascribed to the production of alkaline materials during its growth. Apart from Cd2+, this stain could tolerate Pb2+, Zn2+ and Cu2+ efficiently as well. The resistance decreased in the order of Zn2+, Cd2+>Pb2+>Cu2+>Co2+>Ni2+, Cr2+>Hg2+, Ag+. This strain could grow in the liquid medium with three different inorganic phosphates, and the phosphate solubilization capacity was in the order of Ca3(PO4)2(104 mg/L)>AlPO4 (86 mg/L)>FePO4·4 H2O (17 mg/L).

       

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