Abstract: By using a four-year old Ningqi NO.1 as the tested material, different amounts of biogas liquid manure (0, 6, 8, 10, 12, 14 kg per plant) were applied to study their effects on the growth, quality and yield with a random block experimental design. The results showed that the application of biogas liquid manure not only increased the aboveground biomass, leaf area index and chlorophyll content， but also enhanced the 100-seed mass, yield, polysaccharide, carotenoid, flavonoid and betaine content in the fruits. There were significant variations in different treatments. Spring shoot growth rates increased first and decreased afterwards. Leaf area and biomass peaked under the treatment of 10 kg per plant. With the increase of the application of biogas liquid manure, chlorophyll content presented an obviously rose and tended to be stable when the application of biogas liquid manure was higher than 12 kg per plant. Polysaccharides and betaine content reached maximum value under 10 and 12 kg per plant respectively. Carotenoid content and dried fruit mass increased while flavonoid content did not increase with application of biogas liquid manure, and the dried fruit mass of aplication of biogas liquid manure 10 kg per plant tended to be stable. The combination of the existing fertilization with the application of biogas liquid manure 10 kg per plant had significant effects on the growth, quality and grain yield.