Abstract: All of the 195 clinical isolates of Enterobacteriaceae from chickens were screened for the qnrA by PCR. Susceptibility of qnrA-producing to 8 antimicrobial agents was determined by agar disc diffusion method. Conjugation experiment was performed by using Escherichia coli J53 Az^R as the recipient strain. Southern hybridization was done with plasmid DNA and genetic DNA. IntIⅠwas screened from the qnrA positive strains.The results showed that a qnrA1 gene was identified in a Klebsiella pneumoniae GDKA1. GDKA1 showed resistance to sulfamethoxazole,ampicillin, spectinomycin, chloramphenicol, and tetracycline, susceptible to ciprofloxacin (MIC= 0.019 mg·L^-1) and cefotaxime (MIC=0.047 mg·L^-1) and intermediate to nalidixic acid (MIC= 12 mg·L^-1). A transconjugant was selected during the conjugation experiment and hybridization with qnrA1-specific probes revealed that the qnrA1 gene was located in plasmid DNA.Tranconjugant T_GDKA1 showed a little higher quinolone MIC than E. coli J53 Az^R but not as high as GDKA1. IntI1 was detected in qnrA gene positive strain.In conclusion，qnrA1 gene in K. pneumoniae isolated from health chicken might be transmitted together with class I integron to people through food chain. The presence of qnrA1 gene in health animal commensal bacteria should be noticed.