Abstract: In order to create a procedure for utilization of heterosis in flowering Chinese cabbage, a breeding method for genetic male sterile line was carried out in the experiment. A multiple allele male sterile line of Chinese cabbage‘00S107’was used as a source of male sterility, and recurrent backcrossing method was employed to transfer horticultural characters while the genotype was identified through test cross to transfer the male sterility to a male fertile line ‘Ever-Green’ of flowering Chinese cabbage. A new genetic male sterile line of flowering Chinese cabbage with 100% of male sterile plants and 100% of male sterility ‘GMS203’ was bred. The new male sterile line was applied to develop hybrid combinations. Two excellent combinations were selected through variety compare test. Based on the directional transfer program designed by the research according to the genetic characteristics of the male sterility, the male sterility and horticultural characters could be transferred simultaneously.