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    马伏宁,万树青,刘序铭,赵凤. 黄皮种子中杀松材线虫成分分离及活性测定[J]. 华南农业大学学报, 2009, 30(1). DOI: 10.7671/j.issn.1001-411X.2009.01.007
    引用本文: 马伏宁,万树青,刘序铭,赵凤. 黄皮种子中杀松材线虫成分分离及活性测定[J]. 华南农业大学学报, 2009, 30(1). DOI: 10.7671/j.issn.1001-411X.2009.01.007
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    MA Fu-ning,WAN Shu-qing,LIU Xu-ming,ZHAO Feng. Active Ingredients of Seed Extracts of Clausena lansium and Nematocidal Activity Against Bursaphelenchus xylophilus[J]. Journal of South China Agricultural University, 2009, 30(1). DOI: 10.7671/j.issn.1001-411X.2009.01.007
    Citation: MA Fu-ning,WAN Shu-qing,LIU Xu-ming,ZHAO Feng. Active Ingredients of Seed Extracts of Clausena lansium and Nematocidal Activity Against Bursaphelenchus xylophilus[J]. Journal of South China Agricultural University, 2009, 30(1). DOI: 10.7671/j.issn.1001-411X.2009.01.007
    shu

    黄皮种子中杀松材线虫成分分离及活性测定

    Active Ingredients of Seed Extracts of Clausena lansium and Nematocidal Activity Against Bursaphelenchus xylophilus

      摘要
    • 摘要: 采用药液浸渍法测定了黄皮Clausena lansium不同部位甲醇提取物对松材线虫Bursaphelenchus xylophilus的生物活性,在活性跟踪的基础上,通过萃取、柱层析、薄层制备、重结晶、核磁共振等方法对其有效成分进行了分离和结构鉴定.结果表明,黄皮不同部位甲醇提取物中种子提取物对松材线虫的毒杀活性最高,在1 mg/mL水溶液中处理72 h的校正死亡率为100%.各萃取相中石油醚相的活性最高,从石油醚相中分离、纯化获得一黄色晶体,经核磁共振检测分析为Lansiumamide B(N-甲基-N-顺-苯乙烯-肉桂酰胺).该化合物处理松材线虫24、48、72 h的LC50值分别为8.38、6.36、5.38 mg/L,表明该化合物是黄皮种子中主要杀松材线虫活性成分.

       

      Abstract: Nematocidal activity of methanol extracts from the different parts of Clausena lansium was determined by dipping method.The active ingredients were isolated and identified on the basis of active-directed fractionation with column chromatography,thin layer chromatography(TLC),recrystallization and nuclear magnetic resonance(NMR).The results showed that the most effective extract against Bursaphelenchus xylosphilus was seed methanol extract,which gave 100% mortality after 72 h treatment at the mass concentration of 1 mg/mL.The active ingredients were concentrated in petroleum ether fraction and a yellow crystal was obtained and purified.It was identified as Lansiumamide B(N-methyl-N-cis-styryl-cinnamamide) on the basis of NMR data.The bioassay results revealed that LC50 values of Lansiumamide B against B.xylophilus were 8.38,6.36 and 5.38 mg/L after 24,48 and 72 h treatment,respectively.The results indicated that Lansiumamide B was effective against B.xylophilus.

       

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