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    赵丽,崔保安,陈红英,宋凌云,方忠意,郑兰兰. 海兰鸡白细胞介素18成熟蛋白基因的克隆及在大肠杆菌中的表达[J]. 华南农业大学学报, 2008, 29(1): 88-91. DOI: 10.7671/j.issn.1001-411X.2008.01.022
    引用本文: 赵丽,崔保安,陈红英,宋凌云,方忠意,郑兰兰. 海兰鸡白细胞介素18成熟蛋白基因的克隆及在大肠杆菌中的表达[J]. 华南农业大学学报, 2008, 29(1): 88-91. DOI: 10.7671/j.issn.1001-411X.2008.01.022
    shu
    ZHAO Li,CUI Bao-an,CHEN Hong-ying,SONG Ling-yun,FANG Zhong-yi,ZHENG Lan-lan. Cloning and Expression of Hai Lan Chicken Interleukin 18 in E. coli and Preparation of Its Antiseru[J]. Journal of South China Agricultural University, 2008, 29(1): 88-91. DOI: 10.7671/j.issn.1001-411X.2008.01.022
    Citation: ZHAO Li,CUI Bao-an,CHEN Hong-ying,SONG Ling-yun,FANG Zhong-yi,ZHENG Lan-lan. Cloning and Expression of Hai Lan Chicken Interleukin 18 in E. coli and Preparation of Its Antiseru[J]. Journal of South China Agricultural University, 2008, 29(1): 88-91. DOI: 10.7671/j.issn.1001-411X.2008.01.022
    shu

    海兰鸡白细胞介素18成熟蛋白基因的克隆及在大肠杆菌中的表达

    Cloning and Expression of Hai Lan Chicken Interleukin 18 in E. coli and Preparation of Its Antiseru

      摘要
    • 摘要: 通过RT-PCR方法从用植物血凝素(PHA)活化60日龄海兰鸡的脾脏淋巴细胞中扩增出海兰鸡白细胞介素18(IL-18)成熟蛋白基因的cDNA,并将其克隆到pGEM-T Easy Vector载体上, DNA序列测定表明, 克隆得到的海兰鸡ChIL-18 cDNA基因全序列包括终止密码子在内其编码区大小为510 bp,编码169个氨基酸多肽. 将该基因片段克隆到原核表达载体pQE30构建重组质粒pQE30-ChIL-18,转化大肠杆菌M15,并用IPTG 诱导. 重组菌菌体裂解物SDS-PAGE可检测到相对分子质量为19 000的重组蛋白.

       

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