林瑞庆,庄瑞宏,张雪,林肖玲,宋慧群,朱兴全. 有齿食道口线虫msp基因的克隆与表达[J]. 华南农业大学学报, 2007, 28(4): 91-94. DOI: 10.7671/j.issn.1001-411X.2007.04.022
    引用本文: 林瑞庆,庄瑞宏,张雪,林肖玲,宋慧群,朱兴全. 有齿食道口线虫msp基因的克隆与表达[J]. 华南农业大学学报, 2007, 28(4): 91-94. DOI: 10.7671/j.issn.1001-411X.2007.04.022
    LIN Rui-qing,ZHUANG Rui-hong,ZHANG Xue,LIN Xiao-ling,SONG Hui-qun,ZHU Xing-quan. Cloning and Expression of Major Sperm Protein Gene from Oesophagostomum dentatum[J]. Journal of South China Agricultural University, 2007, 28(4): 91-94. DOI: 10.7671/j.issn.1001-411X.2007.04.022
    Citation: LIN Rui-qing,ZHUANG Rui-hong,ZHANG Xue,LIN Xiao-ling,SONG Hui-qun,ZHU Xing-quan. Cloning and Expression of Major Sperm Protein Gene from Oesophagostomum dentatum[J]. Journal of South China Agricultural University, 2007, 28(4): 91-94. DOI: 10.7671/j.issn.1001-411X.2007.04.022

    有齿食道口线虫msp基因的克隆与表达

    Cloning and Expression of Major Sperm Protein Gene from Oesophagostomum dentatum

    • 摘要: 以有齿食道口线虫为研究对象,扩增出其雄虫的msp基因,克隆至质粒载体pTrcHis-B中,构建了msp基因重组原核表达载体.经测序表明,目的基因msp已以正确的阅读框架整合至表达质粒中.应用大肠杆菌Top10为宿主菌,通过IPTG诱导方法,表达包含msp基因产物的融合蛋白,经SDS-PAGE分析,表明表达蛋白大小正确,表达量高.

       

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