中国3种不同生物型烟粉虱的种群DNA多态性研究
Population Differentiation of Three Biotypes of Bemisia tabaci in China by DNA Polymorphism
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摘要: 利用基因组DNA的多态性研究了中国3种不同生物型烟粉虱的种群分化．烟粉虱种群分别采自北京、扬州和广州的不同寄主上，烟粉虱生物型包括B型、本地型（NaC）和一种新的生物型（Cv型）．对3种生物型烟粉虱的RAPD-PCR结果进行遗传距离聚类分析，结果显示烟粉虱中国本地型和Cv型的种群关系比较近缘，二者与B型种群的亲缘关系较远．3个烟粉虱生物型的种群分化属于种内分化，据此推测Cv型烟粉虱可能起源于中国或其临近地区．Abstract: The differentiation of three haplotypes of Bemisia tabaci populations in China was analyzed using RAPD-PCR in this study.Five B.tabaci populations were collected in the north,middle and south of China,respectively,including three B biotype populations,the indigenous haplotype populations(NaC haplotype) and a new haplotype population from ornamental plants(C_v haplotype).In addition,the greenhouse whitefly,Trialeurodes vaporariorum,was used as a outgroup population in this analysis.The results of the cluster analysis using genetic distances indicated that,first,the population relationship between the NaC haplotype and the C_v haplotype was more close than that between B biotype and NaC haplotype,or that between B biotype and the C_v haplotype;second,the population relationships of the three haplotype of B.tabaci in China was intraspecific,and the C_v haplotype was potentially originated in China or the neighboring countries.
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Keywords:
- Bemisia tabaci /
- population differentiation /
- biotype /
- haplotype /
- DNA polymorphism
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