马现永 曹永长 马静云 毕英佐. 肌肉生成抑制因子多克隆抗体的制备与鉴定[J]. 华南农业大学学报, 2005, 26(3): 89-92. DOI: 10.7671/j.issn.1001-411X.2005.03.025
    引用本文: 马现永 曹永长 马静云 毕英佐. 肌肉生成抑制因子多克隆抗体的制备与鉴定[J]. 华南农业大学学报, 2005, 26(3): 89-92. DOI: 10.7671/j.issn.1001-411X.2005.03.025
    MA Xian-yong,CAO Yong-chang,MA Jing-yun,BI Ying-zuo. Preparation and identification of polyclonal antiserum against myostatin[J]. Journal of South China Agricultural University, 2005, 26(3): 89-92. DOI: 10.7671/j.issn.1001-411X.2005.03.025
    Citation: MA Xian-yong,CAO Yong-chang,MA Jing-yun,BI Ying-zuo. Preparation and identification of polyclonal antiserum against myostatin[J]. Journal of South China Agricultural University, 2005, 26(3): 89-92. DOI: 10.7671/j.issn.1001-411X.2005.03.025

    肌肉生成抑制因子多克隆抗体的制备与鉴定

    Preparation and identification of polyclonal antiserum against myostatin

    • 摘要: 运用纯化的肌肉生成抑制因子(myostatin,简称MSTN)蛋白为免疫原制备弗氏佐剂疫苗,主动免疫健壮的雄性青年家兔以制备抗血清,运用饱和硫酸胺沉淀法对所制备的抗血清成功地进行粗提.Western-Blot和ELISA检测结果是;当抗原质量浓度为20μg/mL,抗体稀释800倍时,所测的P/N仍大于2,证明抗体的制备是成功的,抗体效价为1:12800,且特异性较高.抗体稀释400倍时,血清抗体D450nm值为0.624,P/N值为5.552.

       

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