陈庭,叶庆生,刘伟. 金钗石斛类原球茎诱导及增殖的正交试验[J]. 华南农业大学学报, 2005, 26(3): 60-63. DOI: 10.7671/j.issn.1001-411X.2005.03.017
    引用本文: 陈庭,叶庆生,刘伟. 金钗石斛类原球茎诱导及增殖的正交试验[J]. 华南农业大学学报, 2005, 26(3): 60-63. DOI: 10.7671/j.issn.1001-411X.2005.03.017
    CHEN Ting~1,YE Qing-sheng~2,LIU Wei~3. The orthogonal test of induction and proliferation of Dendrobium nobile protocrom like bodies(PLBs)[J]. Journal of South China Agricultural University, 2005, 26(3): 60-63. DOI: 10.7671/j.issn.1001-411X.2005.03.017
    Citation: CHEN Ting~1,YE Qing-sheng~2,LIU Wei~3. The orthogonal test of induction and proliferation of Dendrobium nobile protocrom like bodies(PLBs)[J]. Journal of South China Agricultural University, 2005, 26(3): 60-63. DOI: 10.7671/j.issn.1001-411X.2005.03.017

    金钗石斛类原球茎诱导及增殖的正交试验

    The orthogonal test of induction and proliferation of Dendrobium nobile protocrom like bodies(PLBs)

    • 摘要: 通过正交试验,筛选了金钗石斛Dendrobiumnobile类原球茎(protocrom likebodies,PLBs)诱导及增殖适宜的培养基.以1/2MS附加8g·L-1琼脂为基本培养基,诱导以NAA、TDZ(噻二唑苯基脲,thidiazuron)、CPPUN(2氯4吡啶基)N1苯脲做三因子四水平试验,增殖则以NAA、BA、蔗糖、pH值做四因子四水平试验,根据L16(45)正交表设计,分别以PLBs诱导率和增质量率为指标,筛选了适宜于PLBs诱导和增殖的培养基.结果显示:1/2MS+30g·L-1蔗糖+8g·L-1琼脂+0.01mg·L-1TDZ+0.005mg·L-1CPPU,pH5.6的培养基适宜于PLBs诱导,1/2MS+20g·L-1蔗糖+8g·L-1琼脂+20g·L-1香蕉汁+1.0mg·L-1NAA+0.2mg·L-1BA,pH5.6的培养基增殖效果较好;诱导率和增质量率分别为46.00%和756.74%.在各因子中,NAA对PLBs诱导率的影响最大,并对PLBs的诱导起抑制作用;蔗糖质量浓度对PLBs增质量率影响最大,且较低的质量浓度更为适宜;筛选出的PLBs诱导和增殖培养基经重复试验,结果相对稳定.

       

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