Abstract: From 2001 to 2002,investigation was carried out on mycoflora of rape petals. (1 908) isolates were obtained from new opened petals and senescent petals which were collected from stages of emerging bloom, full bloom and fall bloom. Except Sclerotinia sclerotiorum, there were other fungi, such as Aureubasidium spp.,Fusarium spp.,Alternaria spp.,Botrytis spp.,Periconia spp.,Aspergillus spp.,Nigrospora spp.,Peniccillium spp.,Trichoderma spp.,Epicoccum spp.. The dominant mycoflra were Aureubasidium spp., Fusarium spp. and Alternaria spp., followed by them were Botrytis spp.,Periconia spp. and Aspergillus spp.. The average isolation frequency of Aureubasidium spp. was the highest, followed by that of Fusarium spp..160 isolates were selected from identified fungi from rape to test their antagonistic effect on S.sclerotiorum by the methods of dual culture.The properties for a antagonist, which were suggested as necessary for S.sclerotiorum, including hyphal interaction, direct parasitism, strong competitive to S.sclerotiorum were observed in vitro. Among them,10 isolates were selected(A4,F6,Au2,As1,-B1,Pe7,AU6,As2,F3,Pe5 ),antagonism and activity of them were strong.