Abstract: Embryogenic callus was successfully induced starting from immature male flowers in 2 out of 5 banana cultivars and starting from scalps in 2 out of 3 cultivars. All these 4 cultivars from which embryogenic callus could be induced belonged to Musa AAA group. The frequency of embryogenic callus induction was depended on genotypes, cultivars and incubation conditions etc. Embryogenic cell suspensions (ECSs) were initiated successfully from embryogenic callus of all these 4 cultivars. The possibility of getting ECSs from embryogenic callus was also cultivar-dependent.