甘蔗不同基因型愈伤组织生化性状的研究

    Biochemical characters of callus in different sugarcane genotypes

    • 摘要: 通过甘蔗心叶的组织培养,研究了11个甘蔗基因型的愈伤组织的10个生化性状的差异,把这些生化性状与甘蔗在大田种植条件下的田间锤度、蔗茎产量进行了相关分析.结果表明,愈伤组织干物质含量、还原糖含量、蔗糖含量、总糖含量、蛋白质含量、酸性转化酶活性、中性转化酶活性、多酚氧化酶活性、过氧化物酶活性、硝酸还原酶活性在不同基因型问都存在显著的差异;愈伤组织的干物质含量与产量有显著的正相关关系,还原糖含量、酸性转化酶活性和多酚氧化酶活性与田间锤度都有显著的负相关关系.

       

      Abstract: A experiment was conducted to find the differences of 10 biochemical characters in callus of 11 sugarcane genotypes, and to find the correlation relationship between these characters and cane yield, Brix under field conditions. The results showed that the contents of dry matter, reducing sugar, sucrose, total sugars and soluble proteins and the activities of acid invertase (AI), neutral invertase(NI), polyphenol oxidase(PPO), peroxidase(POD) and nitrate reductase (NR) were significantly different. The coefficient between the cane yield and the content of dry matter was positive in significant level. There were significant negative correlation relationship between Brix and the reducing sugar content, AI and PPO.

       

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