Abstract: Effect of N-application rate on yield and quality of rice Liangyoupeijiu was studied, in comparison with Shanyou 63 under field conditions. The results showed that the yield increased with the N-application rate, amounted to the greatest level at moderate N level, then descended dramatically at high N level. It is benefit for the nutrition quality, milled quality, steaming and tasty quality to increase N-application rate properly, but not benefit for the head rice rate, chalkiness, amylose and other quality indexes. The difference was significant for the yield and quality between the varieties. Comparing to the rice Shanyou 63, the yield of the rice Liangyoupeijiu was higher and the quality was better at the four nitrogen treatments.