墨兰组织培养中原球茎的形态解剖研究

    • 摘要: 通过对墨兰(Cymidium sinense)组织培养中原球茎外部形态、内部结构的观察研究,发现墨兰茎尖组织培养中产生的原球茎和丛生型原球茎虽外形差别较大,但内部结构都具有根的典型特征。牙的分生组织可以在原球茎表层、丛生型原球茎或其顶端发生。芽的顶端分生组织与随后产生的根的顶端分生组织共同构成胚状结构,其形态与种子胚相似。由于墨兰组培中先后产生的原球茎和丛生型原球茎其内部结构都属典型的根结构,故称

       

      Abstract: The observation on the structure of protocorm of Cymidium sinense shows that inner structures of protocorm and rosette protocorm are similar although their outer forms are different. Buds occur on the protocom and node and end of rosette protocom. Embryoid like structure is formed by shoot apical meristem and root apical meristem and it is similar with seed embryo. According to development and structure, protocom and rosette protocom is considered as a vegetative propagational organ. In addition, the large crystal cells can be observed in the leaf primordia of Cymidium sinense .

       

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