RAPD标记在日本兵库县20个酒米品种类群划分中的应用
The Application of RAPD Technology to Heredity Grouping of 20 Rice Varieties of Hyogo of Japan
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摘要: 应用ＲＡＰＤ分子标记对日本兵库县２０个酒米品种的类群进行了研究。筛选出对不同基因型酒米品种ＤＮＡ扩增具有较好多态性的引物１１个，它们分别是Ａ０２、Ａ０８、Ｂ１８、Ｃ１５、Ｄ０３、Ｄ０８、Ｇ０５、Ｍ１１、Ｑ１６、Ｓ１３和Ｔ０６。根据这些随机引物对不同品种总ＤＮＡ具有特性性的扩增谱带，建立不同品种的ＲＡＰＤ多态性标准模式图，可以快速鉴别品种和进行纯度分析；依据扩增谱带建立０、１型数据，计算２０个酒米品Abstract: Heredity groups were classified by cluster analysis with RAPD technology based on 20 rice varieties of hyogo prefecture of Japan. 11 primers, A 02 , A 08 , B 18 , C 15 , D 03 , D 08 , G 05 , M 11 , Q 16 , S 13 and T 06 , selected from 220 candidate primers act as polymorphic DNA (RAPD) makers for 20 rice. The maps of RAPD standard polymorphic DNA in different varieties can be established by their specific characteristic bands. Thus, the cultivars and their purity could be identified and analysed quickly. In addition, Genetic distance among 20 rice varieties was calculated and clustered with RAPD makers. The results showed that 20 rice varieties could be classified into four groups. The four groups classified by cluster analysis with RAPD technology is consistent with the expected grouping based on available pedigree. The current study indicated that it was practical to group rice with RAPD technology.
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Keywords:
- RAPD maker /
- rice varieties /
- group and cluster classified
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