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    YE Jingjing, ZHANG Fenglin, AI Wei, ZHU Xiaotong, SHU Gang, WANG Lina, GAO Ping, JIANG Qingyan, WANG Songbo. Expression of plasma membrane calcium channel, calcium-sensing receptor and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells[J]. Journal of South China Agricultural University, 2016, 37(5): 7-12. DOI: 10.7671/j.issn.1001-411X.2016.05.002
    Citation: YE Jingjing, ZHANG Fenglin, AI Wei, ZHU Xiaotong, SHU Gang, WANG Lina, GAO Ping, JIANG Qingyan, WANG Songbo. Expression of plasma membrane calcium channel, calcium-sensing receptor and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells[J]. Journal of South China Agricultural University, 2016, 37(5): 7-12. DOI: 10.7671/j.issn.1001-411X.2016.05.002
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    Expression of plasma membrane calcium channel, calcium-sensing receptor and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells

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      • Abstract
    • Objective To investigate the mRNA expression of plasma membrane calcium channel, calcium-sensing receptor (CaSR) and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells (BMSCs).
      Method The porcine BMSCs, isolated and purified from 5-7 d piglet, were induced to adipogenic differentiation. Oil red O staining and triglyceride assay were used to detect the formation of adipocytes. The cells were collected at various time points (0, 1, 2, 5, and 10 d) during the adipogenic differentiation of porcine BMSCs. The mRNA expression patterns of zinc finger protein (Zfp423), preadipocyte factor 1 (Pref-1), bone morphogenetic protein 2 (BMP2), bone morphogenetic protein 4 (BMP4), plasma membrane calcium channel and CaSR genes were detected by real-time quantitative PCR.
      Result The results of oil red O staining and triglyceride assay indicated that porcine BMSCs were successfully induced to adipogenic differentiation. The findings of real-time quantitative PCR demonstrated that the mRNA expression of Zfp423, Pref-1, BMP2 and BMP4 significantly increased at day 5(P < 0.05), suggesting that day 5 is the key time for the adipogenic determination of porcine BMSCs. Meanwhile, the mRNA expression of voltage-dependent alpha-2/delta subunit 1 (CACNA2D1), calcium release-activated calcium channel modulator 1 (Orai1), transient receptor potential canonical type 1 (TRPC1), transient receptor potential melastatin 7 (TRPM7), transient receptor potential vanilloid receptor 1 (TRPV1) and CaSR also significantly increased at day 5, implying that calcium channels and CaSR gene might be involved in the adipogenesis of porcine BMSCs.
      Conclusion Our data reveal the expression patterns of plasma membrane calcium channel, CaSR and adipogenic determination genes.
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