Selection of RAPD Marker for High Lycoppene in Tomato and Its Specific Fragment Cloning

The near isogenic lines of high and low lycoppene processing tomato were used as experiment materials; DNA was extracted from these plants to develop high and low lycoppene DNA gene pools, respectively. RAPD techniques were adopted to screen molecular marker linked to high lycoppene, and only primer S636 was found to be polymorphic between near isogenic lines of high and low lycoppene processing tomato from 260 RAPD random primers. It had been proved that a specific fragment of RAPD marker was linked to high lycoppene in the back cross parent and the single plants in the backcross population. The specific fragment was extracted from the agarose and ligated with pMD-18T vector, then transformed into DH-5α. The positive clone was sequenced to find a 827 bp fragment. This fragment could be used to develop SCAR marker for steady lycoppene.