Cloning and Bioinformatic Analysis of Flagellum flaA Gene of Aeromonas veronii CY0806 from Cyprinus carpio

The flaA gene was amplified from genome template of Aeromonas veronii CY0806 of Cyprinus carpio by PCR. Homogeneous analysis was carried out by comparing the sequencing results with other strains of Aeromonas and bioinformatic analysis was done on the protein coded by flaA. The results showed that flaA Gene with a length of 915 bp, coding 304 amino acids, was obtained; phylogenetic tree analysis results showed that it was on the same branch with other strains of Aeromonas. Bioinformatic analysis results showed that the relative molecular mass of the protein was 32 098.66, pI 5.57, half-life 30 h, unstability index 32.31, fat index 81.05 whereas there was no signal sequence and transmembrane domain. Results of secondary structural analysis showed that the main secondary structures were α-helix and β-sheet. At the same time the tertiary structure was also predicted by homology modeling.