Recombinant Rabies Virus Expressing Hemagglutinin of Avian Influenza Virus

To study the characteristics of rabies virus as vector, hemagglutinin gene of avian influenza virus was inserted at the site of pseudogene between G and L genes and a chimeric virus, designated as HepFHA, was generated by the technique of reverse genetics. The virus HepFHA grew well in BHK-21 cells after passage for 10 times, but did not show hemagglutination. The foreign protein was analyzed by SDS-PAGE and Western-blotting, and the results indicated that the partial protein of hemagglutinin was expressed with relative molecular mass of 39 540, identical to that of HA1.