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    WU Li-jun,HU Kai-lin,WU Zhi-ming. Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus[J]. Journal of South China Agricultural University, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
    Citation: WU Li-jun,HU Kai-lin,WU Zhi-ming. Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus[J]. Journal of South China Agricultural University, 2007, 28(2): 69-72. DOI: 10.7671/j.issn.1001-411X.2007.02.017
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    Transformation of Capsicum annuum with Coat Protein Gene of Cucumber Mosaic Virus

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    • Agrobacterium-mediated transformation of pepper 'B162'with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.Results showed that the best transformation system was: cotyledons explants were precultured for 2 d,and then inoculated with Agrobacterium tumefaciens(D600 nm= 0.3) for 7 min,transferred onto selection differentiation medium after co-cultured for 2 d.10 green adventitious buds with Kan-resistance were screened by Polymerase chain reaction.Two of them were amplified with the band of the fragment of 740 bp.The result showed the CMV-CP gene had been incorporated into the plant genome successfully.
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